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To improve quantitative robustness, the relative concentration of identified compounds was first normalized by the internal standard (d4-alanine, d5-phenylalanine, or d5-tryptophan) based on their correlation with metabolites in the QC samples ( Absolute quantification of metabolite concentration Amino acids, fatty acids, and lactic acids were quantified using chemical standards
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VITAMIN & NUTRIENT INJECTIONS VITAMIN B12 INJECTION Supports energy production, metabolism, and healthy nerve function while helping maintain normal red blood cell formation
We observed no effect of this handling on the survival or growth of wildtype cells, but gspD mutants were highly dependent on copper in the media, confirming that the cells need to express GspD in order to survive and grow (Fig
